This website provides access to all standard protocols and procedures used at the Goodman Lab. We are part of the Department of Public & Ecosystem Health in the College of Veterinary Medicine at Cornell University. Our lab is located at the Baker Institute for Animal Health. If you’re a member of the Goodman Lab, then you can collaboratively edit any of these protocols. This wiki-style approach gives us a central place to develop our lab protocols, while always keeping the most up-to-date protocol in one convenient and publicly accessible place.
If you’re not part of the lab, no worries, you can still peruse the site to see our protocols. Comments and feedback are welcomed! Please reach out to us at (insert emails)
*Template inspired by the Beiting Lab at PennVet Center: https://protocols.hostmicrobe.org/. Please check out their course on RNAseq: https://diytranscriptomics.com/
**Disclaimer (written by Tina herself): the majority of the organization and R tutorials on this page is written by Tina Yu, a Cornell 2023 graduate who spent 4 years in Laura’s lab, working as an undergraduate student first, then as a full-time research assistant. If you know Tina in real life, you would not be surprised by how lengthy some of the explanations are, because as introverted as Tina is, she talks and digresses too much. But she also believes that scientific communication requires thorough explanation since she was once a student who knew nothing. Please bear with her, and she promises she won’t take any feedback personally :)
What is 2nd-generation sequencing/NGS?
Quality Control (QC) and Uploads
bs_cli_v1.6.0 download biosample -i 710163032 -o . --extension=fastq.gzgalaxy-upload --history-name FungalGenomeAssembly S2v3B_S30_L001_R*_001.fastq.gz